- Julia Conner - Unhappy wife who Holmes charmed out of her prior marriage and onto a dissection table.
- Emeline Cigrand - Fiancé who Holmes locked in a vault to suffocate.
- Minnie and Nannie Williams - His wife and sister-in-law from Ft. Worth, Texas.
- Benjamin Pitezel - An assistant Holmes killed in an insurance scam.
- Howard Pitezel - Benjamin's son who Holmes cut into pieces and then burned.
- Alice Pitezel - Benjamin's daughter who Holmes poisoned and stuffed in a chest.
- Nellie Pitezel - Another of Benjamin's daughters who Holmes stuffed in the chest with Alice (Geary).
- Multitude of hotel guests.